제품 > 항체 > 다중클론항체(pAb)

UBE3A Rabbit pAb (A1757)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UBE3A Rabbit pAb (A1757)

Western blot analysis of various lysates, using UBE3A Rabbit pAb (A1757) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - UBE3A Rabbit pAb (A1757)

Immunohistochemistry analysis of paraffin-embedded rat brain using UBE3A Rabbit pAb (A1757) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - UBE3A Rabbit pAb (A1757)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using UBE3A Rabbit pAb (A1757) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - UBE3A Rabbit pAb (A1757)

Immunofluorescence analysis of HeLa cells using UBE3A Rabbit pAb (A1757) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameUBE3A Rabbit pAb
Catalog No.A1757
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human UBE3A (NP_570853.1).
SequenceKLIRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFT
Gene ID
Swiss Prot
SynonymsAS; ANCR; PIX1; E6-AP; HPVE6A; EPVE6AP; UBE3A
Calculated MW101kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, K-562, 293F
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - UBE3A Rabbit pAb (A1757)}

Western blot - UBE3A Rabbit pAb (A1757)

Western blot analysis of various lysates, using UBE3A Rabbit pAb (A1757) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - UBE3A Rabbit pAb (A1757)}

Immunohistochemistry - UBE3A Rabbit pAb (A1757)

Immunohistochemistry analysis of paraffin-embedded rat brain using UBE3A Rabbit pAb (A1757) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - UBE3A Rabbit pAb (A1757)}

Immunohistochemistry - UBE3A Rabbit pAb (A1757)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using UBE3A Rabbit pAb (A1757) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - UBE3A Rabbit pAb (A1757)}

Immunofluorescence - UBE3A Rabbit pAb (A1757)

Immunofluorescence analysis of HeLa cells using UBE3A Rabbit pAb (A1757) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)