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UBE4A Rabbit mAb (A0710)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UBE4A Rabbit mAb (A0710)

Western blot analysis of extracts of HUVEC cells, using UBE4A antibody (A0710) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - UBE4A Rabbit mAb (A0710)

Western blot analysis of extracts of various cell lines, using UBE4A antibody (A0710) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameUBE4A Rabbit mAb
Catalog No.A0710
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2534
This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE4A (Q14139).
SequenceMTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSD
Gene ID
Swiss Prot
SynonymsE4; UFD2; UBOX2; NEDHMS
Calculated MW123kDa
Observed MW125kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPUMC-HUVEC-T1, Mouse brain, Mouse kidney, Rat liver, Rat brain
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - UBE4A Rabbit mAb (A0710)}

Western blot - UBE4A Rabbit mAb (A0710)

Western blot analysis of extracts of HUVEC cells, using UBE4A antibody (A0710) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - UBE4A Rabbit mAb (A0710)}

Western blot - UBE4A Rabbit mAb (A0710)

Western blot analysis of extracts of various cell lines, using UBE4A antibody (A0710) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)