제품 > 항체 > 단일클론항체(mAb)

UCHL3 Rabbit mAb (A1372)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UCHL3 Rabbit mAb (A1372)

Western blot analysis of various lysates using UCHL3 Rabbit mAb (A1372) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - UCHL3 Rabbit mAb (A1372)

Immunohistochemistry analysis of paraffin-embedded rat testis using UCHL3 Rabbit mAb (A1372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - UCHL3 Rabbit mAb (A1372)

Immunohistochemistry analysis of paraffin-embedded human colon using UCHL3 Rabbit mAb (A1372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameUCHL3 Rabbit mAb
Catalog No.A1372
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1874
The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human UCHL3 (NP_005993.1).
SequenceMEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGL
Gene ID
Swiss Prot
SynonymsUCH-L3; UCHL3
Calculated MW26kDa
Observed MW26kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSW620, 293T, PC-12, C2C12, Jurkat, Mouse brain
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

Co-IP(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - UCHL3 Rabbit mAb (A1372)}

    Western blot - UCHL3 Rabbit mAb (A1372)

    Western blot analysis of various lysates using UCHL3 Rabbit mAb (A1372) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - UCHL3 Rabbit mAb (A1372)}

    Immunohistochemistry - UCHL3 Rabbit mAb (A1372)

    Immunohistochemistry analysis of paraffin-embedded rat testis using UCHL3 Rabbit mAb (A1372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - UCHL3 Rabbit mAb (A1372)}

    Immunohistochemistry - UCHL3 Rabbit mAb (A1372)

    Immunohistochemistry analysis of paraffin-embedded human colon using UCHL3 Rabbit mAb (A1372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A1372? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)