제품 > 항체 > 다중클론항체(pAb)

UGT1A9 Rabbit pAb (A1661)

Review (1)Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UGT1A9 Rabbit pAb (A1661)

Western blot analysis of lysates from Mouse liver, using UGT1A9 Rabbit pAb (A1661) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - UGT1A9 Rabbit pAb (A1661)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using UGT1A9 Rabbit pAb (A1661) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Review
ABclonal: review for UGT1A9 Rabbit pAb(A1661)

Overview

Product nameUGT1A9 Rabbit pAb
Catalog No.A1661
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenols.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-250 of human UGT1A9 (NP_066307.1).
SequenceGKLLVVPMDGSHWFTMRSVVEKLILRGHEVVVVMPEVSWQLGRSLNCTVKTYSTSYTLEDLDREFKAFAHAQWKAQVRSIYSLLMGSYNDIFDLFFSNCRSLFKDKKLVEYLKESSFDAVFLDPFDNCGLIVAKYFSLPSVVFARGILCHYLEEGAQCPAPLSYVPRILLGFSDAMTFKERVRNHIMHLEEHLLCHRFFKNALEIASEILQTPVTEYDLYSHTSI
Gene ID
Swiss Prot
SynonymsLUGP4; UDPGT; UGT1I; HLUGP4; UGT-1I; UGT1-9; UGT1.9; UGT1AI; UGT1-09; UGT1A9S; UDPGT 1-9; UGT1A9
Calculated MW60kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse liver
Cellular locationEndoplasmic reticulum membrane, Microsome, Single-pass membrane protein
Customer validation

(Human cells)

IHC(Homo sapiens)

Documents

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ABclonal:Western blot - UGT1A9 Rabbit pAb (A1661)}

Western blot - UGT1A9 Rabbit pAb (A1661)

Western blot analysis of lysates from Mouse liver, using UGT1A9 Rabbit pAb (A1661) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - UGT1A9 Rabbit pAb (A1661)}

Immunohistochemistry - UGT1A9 Rabbit pAb (A1661)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using UGT1A9 Rabbit pAb (A1661) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)