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UPF2 Rabbit pAb (A7091)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - UPF2 Rabbit pAb (A7091)

Western blot analysis of various lysates, using UPF2 Rabbit pAb (A7091) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - UPF2 Rabbit pAb (A7091)

Immunohistochemistry analysis of UPF2 in paraffin-embedded Human kidney tissue using UPF2 Rabbit pAb (A7091) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunoprecipitation - UPF2 Rabbit pAb (A7091)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug UPF2 Rabbit pAb (A7091 1:200). Western blot was performed from the immunoprecipitate using UPF2 Rabbit pAb (A7091) at a dilition of 1:1000.

Overview

Product nameUPF2 Rabbit pAb
Catalog No.A7091
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1153-1272 of human UPF2 (NP_542166.1).
SequenceGPPLGGGEGEAESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR
Gene ID
Swiss Prot
SynonymsHUPF2; RENT2; smg-3; UPF2
Calculated MW148kDa
Observed MW170kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesJurkat, 293T
Cellular locationCytoplasm, perinuclear region

Documents

Certificate of Compliance

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    ABclonal:Western blot - UPF2 Rabbit pAb (A7091)}

    Western blot - UPF2 Rabbit pAb (A7091)

    Western blot analysis of various lysates, using UPF2 Rabbit pAb (A7091) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - UPF2 Rabbit pAb (A7091)}

    Immunohistochemistry - UPF2 Rabbit pAb (A7091)

    Immunohistochemistry analysis of UPF2 in paraffin-embedded Human kidney tissue using UPF2 Rabbit pAb (A7091) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunoprecipitation - UPF2 Rabbit pAb (A7091)}

    Immunoprecipitation - UPF2 Rabbit pAb (A7091)

    Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug UPF2 Rabbit pAb (A7091 1:200). Western blot was performed from the immunoprecipitate using UPF2 Rabbit pAb (A7091) at a dilition of 1:1000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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