제품 > 항체 > 다중클론항체(pAb)

UPF3A Rabbit pAb (A15893)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UPF3A Rabbit pAb (A15893)

Western blot analysis of lysates from mouse testis, using UPF3A Rabbit pAb (A15893) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - UPF3A Rabbit pAb (A15893)

Immunofluorescence analysis of C6 cells using UPF3A Rabbit pAb (A15893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UPF3A Rabbit pAb (A15893)

Immunofluorescence analysis of HeLa cells using UPF3A Rabbit pAb (A15893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UPF3A Rabbit pAb (A15893)

Immunofluorescence analysis of L929 cells using UPF3A Rabbit pAb (A15893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameUPF3A Rabbit pAb
Catalog No.A15893
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Several splice variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 327-476 of human UPF3A (NP_075387.1).
SequenceEGSLEEPQETSHSGSDKEHRDVERSQEQESEAQRYHVDDGRRHRAHHEPERLSRRSEDEQRWGKGPGQDRGKKGSQDSGAPGEAMERLGRAQRCDDSPAPRKERLANKDRPALQLYDPGARFRARECGGNRRICKAEGSGTGPEKREEAE
Gene ID
Swiss Prot
SynonymsUPF3; HUPF3A; RENT3A; UPF3A
Calculated MW55kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse testis
Cellular locationCytoplasm, Nucleus

Documents

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    ABclonal:Western blot - UPF3A Rabbit pAb (A15893)}

    Western blot - UPF3A Rabbit pAb (A15893)

    Western blot analysis of lysates from mouse testis, using UPF3A Rabbit pAb (A15893) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunofluorescence - UPF3A Rabbit pAb (A15893)}

    Immunofluorescence - UPF3A Rabbit pAb (A15893)

    Immunofluorescence analysis of C6 cells using UPF3A Rabbit pAb (A15893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - UPF3A Rabbit pAb (A15893)}

    Immunofluorescence - UPF3A Rabbit pAb (A15893)

    Immunofluorescence analysis of HeLa cells using UPF3A Rabbit pAb (A15893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - UPF3A Rabbit pAb (A15893)}

    Immunofluorescence - UPF3A Rabbit pAb (A15893)

    Immunofluorescence analysis of L929 cells using UPF3A Rabbit pAb (A15893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)