제품 > 항체 > 단일클론항체(mAb)

UQCRH Rabbit mAb (A9395)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UQCRH Rabbit mAb (A9395)

Western blot analysis of various lysates using (A9395) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - UQCRH Rabbit mAb (A9395)

Western blot analysis of various lysates using (A9395) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - UQCRH Rabbit mAb (A9395)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using UQCRH Rabbit mAb (A9395) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - UQCRH Rabbit mAb (A9395)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using UQCRH Rabbit mAb (A9395) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - UQCRH Rabbit mAb (A9395)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using UQCRH Rabbit mAb (A9395) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - UQCRH Rabbit mAb (A9395)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using UQCRH Rabbit mAb (A9395) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameUQCRH Rabbit mAb
Catalog No.A9395
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2724
Predicted to enable ubiquinol-cytochrome-c reductase activity. Predicted to be involved in mitochondrial electron transport, ubiquinol to cytochrome c. Located in mitochondrion.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-91 of human UQCRH (P07919).
SequenceMGLEDEQKMLTESGDPEEEEEEEEELVDPLTTVREQCEQLEKCVKARERLELCDERVSSRSHTEEDCTEELFDFLHARDHCVAHKLFNNLK
Gene ID
Swiss Prot
SynonymsQCR6; UQCR8; MC3DN11; UQCRH
Calculated MW11kDa
Observed MW11kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHT-29, HepG2, SH-SY5Y, Mouse liver, Mouse brain, Mouse heart, Rat skeletal muscle, Rat heart
Cellular locationmitochondrial inner membrane, mitochondrial respirasome, mitochondrial respiratory chain complex III, mitochondrion.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - UQCRH Rabbit mAb (A9395)}

Western blot - UQCRH Rabbit mAb (A9395)

Western blot analysis of various lysates using (A9395) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - UQCRH Rabbit mAb (A9395)}

Western blot - UQCRH Rabbit mAb (A9395)

Western blot analysis of various lysates using (A9395) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - UQCRH Rabbit mAb (A9395)}

Immunohistochemistry - UQCRH Rabbit mAb (A9395)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using UQCRH Rabbit mAb (A9395) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - UQCRH Rabbit mAb (A9395)}

Immunohistochemistry - UQCRH Rabbit mAb (A9395)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using UQCRH Rabbit mAb (A9395) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - UQCRH Rabbit mAb (A9395)}

Immunohistochemistry - UQCRH Rabbit mAb (A9395)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using UQCRH Rabbit mAb (A9395) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - UQCRH Rabbit mAb (A9395)}

Immunohistochemistry - UQCRH Rabbit mAb (A9395)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using UQCRH Rabbit mAb (A9395) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.