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USP10 Rabbit mAb (A4454)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - USP10 Rabbit mAb (A4454)

Western blot analysis of various lysates using USP10 Rabbit mAb (A4454) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - USP10 Rabbit mAb (A4454)

Confocal imaging of Hep G2 cells using USP10 Rabbit mAb (A4454, at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

ABclonal:Immunofluorescence - USP10 Rabbit mAb (A4454)

Confocal imaging of NIH/3T3 cells using USP10 Rabbit mAb (A4454, at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product nameUSP10 Rabbit mAb
Catalog No.A4454
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1015
Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP10 (Q14694).
SequenceMALHSPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDGQEYQRIEFGVDEVIEPSDTLPRTPSYSISSTLNPQAPEFILGCTASKIT
Gene ID
Swiss Prot
SynonymsUBPO; UBP10; MGC2621; USP10
Calculated MW87kDa
Observed MW110kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, 293T
Cellular locationCytoplasm, Early endosome, Nucleus.
Customer validation

WB(Homo sapiens)

IP(Homo sapiens)

IHC(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - USP10 Rabbit mAb (A4454)}

Western blot - USP10 Rabbit mAb (A4454)

Western blot analysis of various lysates using USP10 Rabbit mAb (A4454) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunofluorescence - USP10 Rabbit mAb (A4454)}

Immunofluorescence - USP10 Rabbit mAb (A4454)

Confocal imaging of Hep G2 cells using USP10 Rabbit mAb (A4454, at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
ABclonal:Immunofluorescence - USP10 Rabbit mAb (A4454)}

Immunofluorescence - USP10 Rabbit mAb (A4454)

Confocal imaging of NIH/3T3 cells using USP10 Rabbit mAb (A4454, at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A4454? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)