제품 > 항체 > 다중클론항체(pAb)

USP12 Rabbit pAb (A17862)

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - USP12 Rabbit pAb (A17862)

Western blot analysis of various lysates using USP12 Rabbit pAb (A17862) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 2min.

Overview

Product nameUSP12 Rabbit pAb
Catalog No.A17862
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables cysteine-type endopeptidase activity and thiol-dependent deubiquitinase. Involved in protein deubiquitination. Predicted to be located in nucleoplasm. Predicted to be active in cytosol and nucleus.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP12 (NP_872294.2).
SequenceMEILMTVSKFASICTMGANASALEKEIGPEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKVLAYKSQPRKKESLLTCLADLFHSIATQKKKVGV
Gene ID
Swiss Prot
SynonymsUBH1; USP12L1; USP12
Calculated MW43kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-375, A-549, mouse brain
Cellular locationcytosol, nucleoplasm, nucleus
Customer validation

IP(Mus musculus)

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - USP12 Rabbit pAb (A17862)}

    Western blot - USP12 Rabbit pAb (A17862)

    Western blot analysis of various lysates using USP12 Rabbit pAb (A17862) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 2min.

    * For research use only. Not for therapeutic or diagnostic purposes.

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