제품 > 항체 > 다중클론항체(pAb)

USP12 Rabbit pAb (A17862)

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - USP12 Rabbit pAb (A17862)

Western blot analysis of various lysates using USP12 Rabbit pAb (A17862) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 2min.

Overview

Product nameUSP12 Rabbit pAb
Catalog No.A17862
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables cysteine-type endopeptidase activity and thiol-dependent deubiquitinase. Involved in protein deubiquitination. Predicted to be located in nucleoplasm. Predicted to be active in cytosol and nucleus.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human USP12 (NP_872294.2).
SequenceMEILMTVSKFASICTMGANASALEKEIGPEQFPVNEHYFGLVNFGNTCYCNSVLQALYFCRPFREKVLAYKSQPRKKESLLTCLADLFHSIATQKKKVGV
Gene ID
Swiss Prot
SynonymsUBH1; USP12L1; USP12
Calculated MW43kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-375, A-549, mouse brain
Cellular locationcytosol, nucleoplasm, nucleus
Customer validation

IP(Mus musculus)

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - USP12 Rabbit pAb (A17862)}

    Western blot - USP12 Rabbit pAb (A17862)

    Western blot analysis of various lysates using USP12 Rabbit pAb (A17862) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 2min.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A17862? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)