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USP14 Rabbit pAb (A16643)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - USP14 Rabbit pAb (A16643)

Western blot analysis of various lysates using USP14 Rabbit pAb (A16643) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 2min.

Overview

Product nameUSP14 Rabbit pAb
Catalog No.A16643
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 400-500 of human USP14 (NP_005142.1).
SequenceKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ
Gene ID
Swiss Prot
SynonymsTGT; Ubp6; USP14
Calculated MW56kDa
Observed MW60kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, HeLa, Mouse liver, Mouse brain
Cellular locationcell surface, cytoplasmic vesicle, cytosol, extracellular exosome, plasma membrane

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    ABclonal:Western blot - USP14 Rabbit pAb (A16643)}

    Western blot - USP14 Rabbit pAb (A16643)

    Western blot analysis of various lysates using USP14 Rabbit pAb (A16643) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 2min.

    * For research use only. Not for therapeutic or diagnostic purposes.

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