제품 > 항체 > 다중클론항체(pAb)

USP36 Rabbit pAb (A13115)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - USP36 Rabbit pAb (A13115)

Western blot analysis of various lysates using USP36 Rabbit pAb (A13115) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

Overview

Product nameUSP36 Rabbit pAb
Catalog No.A13115
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the peptidase C19 or ubiquitin-specific protease family of cysteine proteases. Members of this family remove ubiquitin molecules from polyubiquitinated proteins. The encoded protein may deubiquitinate and stabilize the transcription factor c-Myc, also known as MYC, an important oncoprotein known to be upregulated in most human cancers. The encoded protease may also regulate the activation of autophagy. This gene exhibits elevated expression in some breast and lung cancers.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human USP36 (NP_079366.3).
SequenceMPIVDKLKEALKPGRKDSADDGELGKLLASSAKKVLLQKIEFEPASKSFSYQLEALKSKYVLLNPKTEGASRHKSGDDPPARRQGSEHTYESCGDGVPAPQKVLFPTERLSLRWERVFRV
Gene ID
Swiss Prot
SynonymsDUB1; USP36
Calculated MW123kDa
Observed MW123kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, A-431, HeLa
Cellular locationNucleus, nucleolus
ABclonal:Western blot - USP36 Rabbit pAb (A13115)}

Western blot - USP36 Rabbit pAb (A13115)

Western blot analysis of various lysates using USP36 Rabbit pAb (A13115) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)