제품 > 항체 > 다중클론항체(pAb)

USP44 Rabbit pAb (A15528)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - USP44 Rabbit pAb (A15528)

Western blot analysis of various lysates using USP44 Rabbit pAb (A15528) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameUSP44 Rabbit pAb
Catalog No.A15528
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset. This protein specifically deubiquitinates CDC20, which stabilizes the anaphase promoting complex/cyclosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-270 of human USP44 (NP_115523.2).
SequenceRTLSAIKSQNYHCTTRSGRFLRSMGTGDDSYFLHDGAQSLLQSEDQLYTALWHRRRILMGKIFRTWFEQSPIGRKKQEEPFQEKIVVKREVKKRRQELEYQVKAELESMPPRKSLRLQGLAQSTIIEIVSVQVPAQTPASPAKDKVLSTSENEISQKVSDSSVKRRPIVTP
Gene ID
Swiss Prot
SynonymsUSP44
Calculated MW81kDa
Observed MW81kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, Raji, HT-29, Mouse lung, Mouse pancreas, Mouse liver, Rat stomach, Rat kidney
Cellular locationNucleus
    ABclonal:Western blot - USP44 Rabbit pAb (A15528)}

    Western blot - USP44 Rabbit pAb (A15528)

    Western blot analysis of various lysates using USP44 Rabbit pAb (A15528) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)