제품 > 항체 > 다중클론항체(pAb)

UVRAG Rabbit pAb (A8462)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UVRAG Rabbit pAb (A8462)

Western blot analysis of various lysates using UVRAG Rabbit pAb (A8462) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - UVRAG Rabbit pAb (A8462)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using UVRAG Rabbit pAb (A8462) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - UVRAG Rabbit pAb (A8462)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using UVRAG Rabbit pAb (A8462) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - UVRAG Rabbit pAb (A8462)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using UVRAG Rabbit pAb (A8462) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - UVRAG Rabbit pAb (A8462)

Immunohistochemistry analysis of paraffin-embedded Rat brain using UVRAG Rabbit pAb (A8462) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameUVRAG Rabbit pAb
Catalog No.A8462
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 501-699 of human UVRAG (NP_003360.2).
SequenceKGHRKRASSENERLQYKTPPPSYNSALAQPVTTVPSMGETERKITSLSSSLDTSLDFSKENKKKGEDLVGSLNGGHANVHPSQEQGEALSGHRATVNGTLLPSEQAGSASVQLPGEFHPVSEAELCCTVEQAEEIIGLEATGFASGDQLEAFNCIPVDSAVAVECDEQVLGEFEEFSRRIYALNENVSSFRRPRRSSDK
Gene ID
Swiss Prot
Synonymsp63; DHTX; VPS38; UVRAG
Calculated MW78kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesLO2, HeLa, Mouse liver, Rat liver, Rat brain
Cellular locationChromosome, Early endosome, Endoplasmic reticulum, Late endosome, Lysosome, Midbody, centromere
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - UVRAG Rabbit pAb (A8462)}

Western blot - UVRAG Rabbit pAb (A8462)

Western blot analysis of various lysates using UVRAG Rabbit pAb (A8462) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - UVRAG Rabbit pAb (A8462)}

Immunohistochemistry - UVRAG Rabbit pAb (A8462)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using UVRAG Rabbit pAb (A8462) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - UVRAG Rabbit pAb (A8462)}

Immunohistochemistry - UVRAG Rabbit pAb (A8462)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using UVRAG Rabbit pAb (A8462) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - UVRAG Rabbit pAb (A8462)}

Immunohistochemistry - UVRAG Rabbit pAb (A8462)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using UVRAG Rabbit pAb (A8462) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - UVRAG Rabbit pAb (A8462)}

Immunohistochemistry - UVRAG Rabbit pAb (A8462)

Immunohistochemistry analysis of paraffin-embedded Rat brain using UVRAG Rabbit pAb (A8462) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A8462? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)