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VAPB Rabbit pAb (A5363)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VAPB Rabbit pAb (A5363)

Western blot analysis of various lysates using VAPB Rabbit pAb (A5363) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - VAPB Rabbit pAb (A5363)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using VAPB Rabbit pAb (A5363) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VAPB Rabbit pAb (A5363)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using VAPB Rabbit pAb (A5363) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - VAPB Rabbit pAb (A5363)

Immunofluorescence analysis of U2OS cells using VAPB Rabbit pAb (A5363) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameVAPB Rabbit pAb
Catalog No.A5363
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 10-222 of human VAPB (NP_004729.1).
SequenceLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVKTTAPRRYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFMVQSMFAPTDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTASKTETPIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDGLRMRKTVQSNSPISALAPTGKEEGLST
Gene ID
Swiss Prot
SynonymsALS8; VAP-B; VAMP-B; VAPB
Calculated MW27kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse lung, Mouse brain, Mouse kidney, Rat heart
Cellular locationEndoplasmic reticulum membrane, Single-pass type IV membrane protein
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - VAPB Rabbit pAb (A5363)}

    Western blot - VAPB Rabbit pAb (A5363)

    Western blot analysis of various lysates using VAPB Rabbit pAb (A5363) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - VAPB Rabbit pAb (A5363)}

    Immunohistochemistry - VAPB Rabbit pAb (A5363)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using VAPB Rabbit pAb (A5363) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - VAPB Rabbit pAb (A5363)}

    Immunohistochemistry - VAPB Rabbit pAb (A5363)

    Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using VAPB Rabbit pAb (A5363) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - VAPB Rabbit pAb (A5363)}

    Immunofluorescence - VAPB Rabbit pAb (A5363)

    Immunofluorescence analysis of U2OS cells using VAPB Rabbit pAb (A5363) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A5363? Please let us know so that we can cite the reference in this datasheet.

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    Secondary Antibodies (26)