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VCP Rabbit pAb (A13368)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VCP Rabbit pAb (A13368)

Western blot analysis of various lysates using VCP Rabbit pAb (A13368) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - VCP Rabbit pAb (A13368)

Immunohistochemistry analysis of paraffin-embedded human placenta using VCP Rabbit pAb (A13368) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VCP Rabbit pAb (A13368)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using VCP Rabbit pAb (A13368) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VCP Rabbit pAb (A13368)

Immunohistochemistry analysis of paraffin-embedded rat kidney using VCP Rabbit pAb (A13368) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - VCP Rabbit pAb (A13368)

Immunofluorescence analysis of C6 cells using VCP Rabbit pAb (A13368) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - VCP Rabbit pAb (A13368)

Immunofluorescence analysis of U-2 OS cells using VCP Rabbit pAb (A13368) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameVCP Rabbit pAb
Catalog No.A13368
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 647-806 of human VCP (NP_009057.1).
SequenceLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG
Gene ID
Swiss Prot
Synonymsp97; TERA; CDC48; FTDALS6; VCP
Calculated MW89kDa
Observed MW90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, K-562, SH-SY5Y, Mouse brain, Mouse spleen, Rat liver
Cellular locationCytoplasm, Endoplasmic reticulum, Nucleus, cytosol
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - VCP Rabbit pAb (A13368)}

Western blot - VCP Rabbit pAb (A13368)

Western blot analysis of various lysates using VCP Rabbit pAb (A13368) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - VCP Rabbit pAb (A13368)}

Immunohistochemistry - VCP Rabbit pAb (A13368)

Immunohistochemistry analysis of paraffin-embedded human placenta using VCP Rabbit pAb (A13368) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - VCP Rabbit pAb (A13368)}

Immunohistochemistry - VCP Rabbit pAb (A13368)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using VCP Rabbit pAb (A13368) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - VCP Rabbit pAb (A13368)}

Immunohistochemistry - VCP Rabbit pAb (A13368)

Immunohistochemistry analysis of paraffin-embedded rat kidney using VCP Rabbit pAb (A13368) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - VCP Rabbit pAb (A13368)}

Immunofluorescence - VCP Rabbit pAb (A13368)

Immunofluorescence analysis of C6 cells using VCP Rabbit pAb (A13368) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VCP Rabbit pAb (A13368)}

Immunofluorescence - VCP Rabbit pAb (A13368)

Immunofluorescence analysis of U-2 OS cells using VCP Rabbit pAb (A13368) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A13368? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)