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VIP Rabbit mAb (A24164)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - VIP Rabbit mAb (A24164)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with VIP using VIP Rabbit mAb (A24164) at 1:5000 dilution. 
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunofluorescence - VIP Rabbit mAb (A24164)

Confocal imaging of 293T cells transfected with VIP using VIP Rabbit mAb (A24164, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameVIP Rabbit mAb
Catalog No.A24164
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65218
The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 31-170 of human VIP (NP_003372.1).
SequenceAPSALRLGDRIPFEGANEPDQVSLKEDIDMLQNALAENDTPYYDVSRNARHADGVFTSDFSKLLGQLSAKKYLESLMGKRVSSNISEDPVPVKRHSDAVFTDNYTRLRKQMAVKKYLNSILNGKRSSEGESPDFPEELEK
Gene ID
Swiss Prot
SynonymsPHM27; VIP
Calculated MW19kDa
Observed MW19kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:20000
  • IF/ICC 1:300 - 1:1200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T transfected with VIP (Human)
Cellular locationSecreted.

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    ABclonal:Western blot - VIP Rabbit mAb (A24164)}

    Western blot - VIP Rabbit mAb (A24164)

    Western blot analysis of lysates from wild type (WT) and 293T cells transfected with VIP using VIP Rabbit mAb (A24164) at 1:5000 dilution. 
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.
    ABclonal:Immunofluorescence - VIP Rabbit mAb (A24164)}

    Immunofluorescence - VIP Rabbit mAb (A24164)

    Confocal imaging of 293T cells transfected with VIP using VIP Rabbit mAb (A24164, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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