제품 > 항체 > 다중클론항체(pAb)

VLDLR Rabbit pAb (A7345)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of extracts of various cell lines, using VLDLR antibody (A7345) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Overview

Product name	VLDLR Rabbit pAb
Catalog No.	A7345
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 833-869 of human VLDLR (NP_003374.3).
Sequence	DNPVYLKTTEEDLSIDIGRHSASVGHTYPAISVVSTD
Gene ID
Swiss Prot
Synonyms	CAMRQ1; CARMQ1; CHRMQ1; VLDL-R; VLDLRCH
Calculated MW	96kDa
Observed MW	110kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	THP-1, Mouse lung, Mouse ovary
Cellular location	Membrane, Single-pass type I membrane protein, clathrin-coated pit
Customer validation	IHC(Gallus gallus)