제품 > 항체 > 다중클론항체(pAb)

VMP1 Rabbit pAb (A15523)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VMP1 Rabbit pAb (A15523)

Western blot analysis of lysates from mouse kidney, using VMP1 Rabbit pAb (A15523) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - VMP1 Rabbit pAb (A15523)

Immunofluorescence analysis of NIH-3T3 cells using VMP1 Rabbit pAb (A15523) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - VMP1 Rabbit pAb (A15523)

Immunofluorescence analysis of U-2 OS cells using VMP1 Rabbit pAb (A15523) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameVMP1 Rabbit pAb
Catalog No.A15523
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transmembrane protein that plays a key regulatory role in the process of autophagy. The ectopic overexpression of the encoded protein in cultured cells triggers autophagy even under nutrient-rich conditions. This gene is overexpressed in pancreatitis affected acinar cells where the encoded protein mediates sequestration and degradation of potentially deleterious activated zymogen granules in a process termed, zymophagy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human VMP1 (NP_112200.2).
SequenceKMHIQKIFVIITFSKHIVEQMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGTPQGENWLSWMFEKLVVVMVCYFILSIINSMAQSYAKRIQQRLNSEEKTK
Gene ID
Swiss Prot
SynonymsEPG3; TANGO5; TMEM49; VMP1
Calculated MW46kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse kidney
Cellular locationCell membrane, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment membrane, Multi-pass membrane protein, Vacuole membrane

Documents

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ABclonal:Western blot - VMP1 Rabbit pAb (A15523)}

Western blot - VMP1 Rabbit pAb (A15523)

Western blot analysis of lysates from mouse kidney, using VMP1 Rabbit pAb (A15523) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - VMP1 Rabbit pAb (A15523)}

Immunofluorescence - VMP1 Rabbit pAb (A15523)

Immunofluorescence analysis of NIH-3T3 cells using VMP1 Rabbit pAb (A15523) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VMP1 Rabbit pAb (A15523)}

Immunofluorescence - VMP1 Rabbit pAb (A15523)

Immunofluorescence analysis of U-2 OS cells using VMP1 Rabbit pAb (A15523) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)