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VPS11 Rabbit mAb (A19798)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - VPS11 Rabbit mAb (A19798)

Western blot analysis of various lysates using VPS11 Rabbit mAb (A19798) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - VPS11 Rabbit mAb (A19798)

Western blot analysis of various lysates using VPS11 Rabbit mAb (A19798) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - VPS11 Rabbit mAb (A19798)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using VPS11 Rabbit mAb (A19798) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VPS11 Rabbit mAb (A19798)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using VPS11 Rabbit mAb (A19798) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameVPS11 Rabbit mAb
Catalog No.A19798
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2325
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human VPS11 (Q9H270).
SequenceMAAYLQWRRFVFFDKELVKEPLSNDGAAPGATPASGSAASKFLCLPPGITVCDSGRGSLVFGDMEGQIWFLPRSLQLTGFQAYKLRVTHLYQLKQHNILA
Gene ID
Swiss Prot
SynonymsEND1; PEP5; HLD12; RNF108; hVPS11; DYT32; VPS11
Calculated MW108kDa
Observed MW108kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, A-431, Raji, Mouse testis, Mouse brain
Cellular locationautophagosome, clathrin-coated vesicle, CORVET complex, early endosome, endosome, late endosome, lysosome

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - VPS11 Rabbit mAb (A19798)}

    Western blot - VPS11 Rabbit mAb (A19798)

    Western blot analysis of various lysates using VPS11 Rabbit mAb (A19798) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - VPS11 Rabbit mAb (A19798)}

    Western blot - VPS11 Rabbit mAb (A19798)

    Western blot analysis of various lysates using VPS11 Rabbit mAb (A19798) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - VPS11 Rabbit mAb (A19798)}

    Immunohistochemistry - VPS11 Rabbit mAb (A19798)

    Immunohistochemistry analysis of paraffin-embedded human lung cancer using VPS11 Rabbit mAb (A19798) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - VPS11 Rabbit mAb (A19798)}

    Immunohistochemistry - VPS11 Rabbit mAb (A19798)

    Immunohistochemistry analysis of paraffin-embedded mouse stomach using VPS11 Rabbit mAb (A19798) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.