제품 > 항체 > 다중클론항체(pAb)

VPS13A Rabbit pAb (A17419)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - VPS13A Rabbit pAb (A17419)

Western blot analysis of lysates from U-87MG cells, using VPS13A Rabbit pAb (A17419) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameVPS13A Rabbit pAb
Catalog No.A17419
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene may control steps in the cycling of proteins through the trans-Golgi network to endosomes, lysosomes and the plasma membrane. Mutations in this gene cause the autosomal recessive disorder, chorea-acanthocytosis. Alternative splicing of this gene results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 350-600 of human VPS13A (NP_150648.2).
SequenceWSWKHIRKHRQKVKQYKELYKKKLTSKKPPGELLVSLEELEKTLDVFNITIARQTAEVEVKKAGYKIYKEGVKDPEDNKGWFSWLWSWSEQNTNEQQPDVQPETLEEMLTPEEKALLYEAIGYSETAVDPTLLKTFEALKFFVHLKSMSIVLRENHQKPELVDIVIEEFSTLIVQRPGAQAIKFETKIDSFHITGLPDNSEKPRLLSSLDDAMSLFQITFEINPLDETVSQRCIIEAEPLEIIYDARTVNS
Gene ID
Swiss Prot
SynonymsCHAC; BLTP5A; CHOREIN; VPS13A
Calculated MW360kDa
Observed MW360kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG
Cellular locationcytosol, Golgi apparatus, lipid droplet, mitochondrial membrane, mitochondrial outer membrane
    ABclonal:Western blot - VPS13A Rabbit pAb (A17419)}

    Western blot - VPS13A Rabbit pAb (A17419)

    Western blot analysis of lysates from U-87MG cells, using VPS13A Rabbit pAb (A17419) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)