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VPS33B Rabbit pAb (A8799)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - VPS33B Rabbit pAb (A8799)

Western blot analysis of extracts of various cell lines, using VPS33B antibody (A8799) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameVPS33B Rabbit pAb
Catalog No.A8799
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 348-617 of human VPS33B (NP_061138.3).
SequenceCESIMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFGGAYVPLSCRIIEQVLERRSWQGLDEVVRLLNCSDFAFTDMTKEDKASSESLRLILVVFLGGCTFSEISALRFLGREKGYRFIFLTTAVTNSARLMEAMSEVKA
Gene ID
Swiss Prot
SynonymsKDIDAR; PFIC12
Calculated MW71kDa
Observed MW62kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-549, MCF7, DU145
Cellular locationCytoplasmic side, Cytoplasmic vesicle, Early endosome, Late endosome membrane, Lysosome membrane, Peripheral membrane protein, Recycling endosome, clathrin-coated vesicle

Documents

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    ABclonal:Western blot - VPS33B Rabbit pAb (A8799)}

    Western blot - VPS33B Rabbit pAb (A8799)

    Western blot analysis of extracts of various cell lines, using VPS33B antibody (A8799) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)