제품 > 항체 > 다중클론항체(pAb)

WBP2NL Rabbit pAb (A18555)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - WBP2NL Rabbit pAb (A18555)

Western blot analysis of lysates from Rat spleen, using WBP2NL Rabbit pAb (A18555) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameWBP2NL Rabbit pAb
Catalog No.A18555
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
WBP2NL is a sperm-specific WW domain-binding protein that promotes meiotic resumption and pronuclear development during oocyte fertilization (Wu et al., 2007 [PubMed 17289678]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human WBP2NL (NP_689826.2).
SequenceMAVNQSHTENRRGALIPNGESLLKRSPNVELSFPQRSEGSNVFSGRKTGTLFLTSYRVIFITSCSISDPMLSFMMPFDLMTNLTVEQPVFAANFIKGTIQAAPYGGWEGQATFKLVFRNG
Gene ID
Swiss Prot
SynonymsPAWP; GRAMD7; WBP2NL
Calculated MW32kDa
Observed MW32kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat spleen
Cellular locationnucleus, perinuclear theca, sperm flagellum

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    ABclonal:Western blot - WBP2NL Rabbit pAb (A18555)}

    Western blot - WBP2NL Rabbit pAb (A18555)

    Western blot analysis of lysates from Rat spleen, using WBP2NL Rabbit pAb (A18555) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)