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WDR61 Rabbit pAb (A15520)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - WDR61 Rabbit pAb (A15520)

Western blot analysis of various lysates using WDR61 Rabbit pAb (A15520) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.

ABclonal:Immunohistochemistry - WDR61 Rabbit pAb (A15520)

Immunohistochemistry analysis of paraffin-embedded Human placenta using WDR61 Rabbit pAb (A15520) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - WDR61 Rabbit pAb (A15520)

Immunohistochemistry analysis of paraffin-embedded Rat brain using WDR61 Rabbit pAb (A15520) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameWDR61 Rabbit pAb
Catalog No.A15520
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human WDR61 (NP_079510.1).
SequenceMTNQYGILFKQEQAHDDAIWSVAWGTNKKENSETVVTGSLDDLVKVWKWRDERLDLQWSLEGHQLGVVSVDISHTLPIAASSSLDAHIRLWDLENGKQIKSIDAGPVDAWTLAFSPDSQYLATGTHVGKVNIFGVESGKKEYSLDTRGKFILSIAYSPDGKYLASGAIDGIINIFDIATGKLLHTLEGHAMPIRSLTFSPDSQLLVTASDDGYIKIYDVQHANLAGTLSGHASWVLNVAFCPDDTHFVSSSSDKSVKVWDVGTRTCVHTFFDHQDQVWGVKYNGNGSKIVSVGDDQEIHIYDCPI
Gene ID
Swiss Prot
SynonymsSKI8; REC14; WDR61
Calculated MW34kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, A-549, mouse stomach, mouse brain, mouse kidney, rat pancreas
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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    ABclonal:Western blot - WDR61 Rabbit pAb (A15520)}

    Western blot - WDR61 Rabbit pAb (A15520)

    Western blot analysis of various lysates using WDR61 Rabbit pAb (A15520) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 40s.
    ABclonal:Immunohistochemistry - WDR61 Rabbit pAb (A15520)}

    Immunohistochemistry - WDR61 Rabbit pAb (A15520)

    Immunohistochemistry analysis of paraffin-embedded Human placenta using WDR61 Rabbit pAb (A15520) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - WDR61 Rabbit pAb (A15520)}

    Immunohistochemistry - WDR61 Rabbit pAb (A15520)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using WDR61 Rabbit pAb (A15520) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.