제품 > 항체 > 단일클론항체(mAb)

WIF1 Rabbit mAb (A24119)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - WIF1 Rabbit mAb (A24119)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with WIF1 using WIF1 Rabbit mAb (A24119) at 1:7000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): 293T.
Exposure time: 10s.

ABclonal:Immunofluorescence - WIF1 Rabbit mAb (A24119)

Confocal imaging of paraffin-embedded Mouse heart using WIF1 Rabbit mAb (A24119, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameWIF1 Rabbit mAb
Catalog No.A24119
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63858
The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 29-379 of human WIF1 (NP_009122.2).
SequenceGPPQEESLYLWIDAHQARVLIGFEEDILIVSEGKMAPFTHDFRKAQQRMPAIPVNIHSMNFTWQAAGQAEYFYEFLSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEGNTILQTPQNAIFFKTCQQAECPGGCRNGGFCNERRICECPDGFHGPHCEKALCTPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCEISKCPQPCRNGGKCIGKSKCKCSKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPPESNYIW
Gene ID
Swiss Prot
SynonymsWIF-1; WIF1
Calculated MW42kDa
Observed MW41kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:7000 - 1:28000
  • IF/ICC 1:200 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T transfected with WIF1 (Human)
Cellular locationSecreted.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - WIF1 Rabbit mAb (A24119)}

Western blot - WIF1 Rabbit mAb (A24119)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with WIF1 using WIF1 Rabbit mAb (A24119) at 1:7000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): 293T.
Exposure time: 10s.
ABclonal:Immunofluorescence - WIF1 Rabbit mAb (A24119)}

Immunofluorescence - WIF1 Rabbit mAb (A24119)

Confocal imaging of paraffin-embedded Mouse heart using WIF1 Rabbit mAb (A24119, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

재조합 단백질 (1)

Secondary Antibodies (26)