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WIF1 Rabbit pAb (A5386)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - WIF1 Rabbit pAb (A5386)

Western blot analysis of HeLa, using WIF1 antibody (A5386) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunofluorescence - WIF1 Rabbit pAb (A5386)

Immunofluorescence analysis of NIH/3T3 cells using WIF1 Rabbit pAb (A5386) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - WIF1 Rabbit pAb (A5386)

Immunofluorescence analysis of PC-12 cells using WIF1 Rabbit pAb (A5386) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameWIF1 Rabbit pAb
Catalog No.A5386
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 307-377 of human WIF1 (NP_009122.2).
SequenceKPVCEPGCGAHGTCHEPNKCQCQEGWHGRHCNKRYEASLIHALRPAGAQLRQHTPSLKKAEERRDPPESNY
Gene ID
Swiss Prot
SynonymsWIF-1
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular locationSecreted

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ABclonal:Western blot - WIF1 Rabbit pAb (A5386)}

Western blot - WIF1 Rabbit pAb (A5386)

Western blot analysis of HeLa, using WIF1 antibody (A5386) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunofluorescence - WIF1 Rabbit pAb (A5386)}

Immunofluorescence - WIF1 Rabbit pAb (A5386)

Immunofluorescence analysis of NIH/3T3 cells using WIF1 Rabbit pAb (A5386) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - WIF1 Rabbit pAb (A5386)}

Immunofluorescence - WIF1 Rabbit pAb (A5386)

Immunofluorescence analysis of PC-12 cells using WIF1 Rabbit pAb (A5386) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)