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WNT5A Rabbit pAb (A12744)

Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - WNT5A Rabbit pAb (A12744)

Western blot analysis of various lysates using (A12744) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - WNT5A Rabbit pAb (A12744)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using WNT5A Rabbit pAb (A12744) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - WNT5A Rabbit pAb (A12744)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using WNT5A Rabbit pAb (A12744) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - WNT5A Rabbit pAb (A12744)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using WNT5A Rabbit pAb (A12744) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - WNT5A Rabbit pAb (A12744)

Immunofluorescence analysis of PC-12 cells using WNT5A Rabbit pAb (A12744) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameWNT5A Rabbit pAb
Catalog No.A12744
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 116-215 of human WNT5A (NP_003383.4).
SequenceSTVDNTSVFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELSTCGCSRAARPKDLPRDWLWGGCGDNIDYGYRFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTTQDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSE
Gene ID
Swiss Prot
SynonymshWNT5A; WNT5A
Calculated MW42kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSKOV3, Rat lung
Cellular locationSecreted, extracellular matrix, extracellular space.
Customer validation

IF(Homo sapiens, Mus musculus)

WB(Mus musculus, Homo sapiens)

IHC(Homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - WNT5A Rabbit pAb (A12744)}

Western blot - WNT5A Rabbit pAb (A12744)

Western blot analysis of various lysates using (A12744) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - WNT5A Rabbit pAb (A12744)}

Immunohistochemistry - WNT5A Rabbit pAb (A12744)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using WNT5A Rabbit pAb (A12744) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - WNT5A Rabbit pAb (A12744)}

Immunohistochemistry - WNT5A Rabbit pAb (A12744)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using WNT5A Rabbit pAb (A12744) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - WNT5A Rabbit pAb (A12744)}

Immunohistochemistry - WNT5A Rabbit pAb (A12744)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using WNT5A Rabbit pAb (A12744) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - WNT5A Rabbit pAb (A12744)}

Immunofluorescence - WNT5A Rabbit pAb (A12744)

Immunofluorescence analysis of PC-12 cells using WNT5A Rabbit pAb (A12744) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A12744? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

ELISA 키트 (1)

Secondary Antibodies (26)