제품 > 항체 > 다중클론항체(pAb)

Xa13 Rabbit pAb (A20630)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - Xa13 Rabbit pAb (A20630)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using Xa13 Rabbit pAb (A20630) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameXa13 Rabbit pAb
Catalog No.A20630
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Mediates both low-affinity uptake and efflux of sugar across the plasma membrane. Required for pollen viability. Involved in the transport of copper, in cooperation with COPT1 and COPT2.Confers sensitivity to bacterial blight mediated by X.oryzae pv. oryzae (Xoo) in its Xa13 allelic form (e.g. cv. IR24), probably by providing the sugar required for the pathogen growth, or by reducing copper contents in xylem. However, a recessive resistance can be associated with the xa13 allele (in which the promoter is mutated leading to reduced induction upon pathogen infection, e.g. cv. IRBB13), specifically toward Xoo Philippine race 6 and Indian race PXO8.
ImmunogenRecombinant protein of Oryza sativa Xa13.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsXA13; Os8N3; SWEET11; OsJ_28080; OsSWEET11; Xa13
Calculated MW33kDa
Observed MW40kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesleaves
Cellular locationplasma membrane
    ABclonal:Western blot - Xa13 Rabbit pAb (A20630)}

    Western blot - Xa13 Rabbit pAb (A20630)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using Xa13 Rabbit pAb (A20630) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)