제품 > 항체 > 다중클론항체(pAb)

YARS Rabbit pAb (A6791)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - YARS Rabbit pAb (A6791)

Western blot analysis of various lysates using YARS Rabbit pAb (A6791) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunoprecipitation - YARS Rabbit pAb (A6791)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg YARS antibody (A6791). Western blot was performed from the immunoprecipitate using YARS antibody (A6791) at a dilution of 1:1000.

Overview

Product nameYARS Rabbit pAb
Catalog No.A6791
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human YARS (NP_003671.1).
SequenceMGDAPSPEEKLHLITRNLQEVLGEEKLKEILKERELKIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVTILFADLHAYLDNMKAPWELLELRVSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVVTQHDSKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGIDQRKIFTFAEKYLPALGYSKRVHLMNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPLKSEFVILRDEKWGGNKTYTAYVDLEKDFA
Gene ID
Swiss Prot
SynonymsYRS; YTS; YARS; TYRRS; CMTDIC; IMNEPD2
Calculated MW59kDa
Observed MW59kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesHeLa, SKOV3, Mouse brain, Mouse skeletal muscle, Rat brain
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - YARS Rabbit pAb (A6791)}

Western blot - YARS Rabbit pAb (A6791)

Western blot analysis of various lysates using YARS Rabbit pAb (A6791) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunoprecipitation - YARS Rabbit pAb (A6791)}

Immunoprecipitation - YARS Rabbit pAb (A6791)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg YARS antibody (A6791). Western blot was performed from the immunoprecipitate using YARS antibody (A6791) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6791? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)