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YKL-40/CHI3L1 Rabbit mAb (A20792)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - YKL-40/CHI3L1 Rabbit mAb (A20792)

Western blot analysis of lysates from THP-1 cells, using YKL-40/CHI3L1 Rabbit mAb (A20792) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - YKL-40/CHI3L1 Rabbit mAb (A20792)

Western blot analysis of various lysates using YKL-40/CHI3L1 Rabbit mAb (A20792) at1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - YKL-40/CHI3L1 Rabbit mAb (A20792)

Confocal imaging of THP-1 cells using YKL-40/CHI3L1 Rabbit mAb (A20792, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - YKL-40/CHI3L1 Rabbit mAb (A20792)

Confocal imaging of paraffin-embedded Rat brain tissue using YKL-40/CHI3L1 Rabbit mAb (A20792, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameYKL-40/CHI3L1 Rabbit mAb
Catalog No.A20792
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC51312
Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 284-383 of human YKL-40/CHI3L1 (NP_001267.2).
SequencePGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT
Gene ID
Swiss Prot
SynonymsGP39; ASRT7; GP-39; YK-40; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39; YKL-40/CHI3L1
Calculated MW43kDa
Observed MW30-43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesTHP-1, U-87MG, Mouse lung
Cellular locationCytoplasm, Endoplasmic reticulum, Secreted, extracellular space, perinuclear region.
Customer validation

WB(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - YKL-40/CHI3L1 Rabbit mAb (A20792)}

    Western blot - YKL-40/CHI3L1 Rabbit mAb (A20792)

    Western blot analysis of lysates from THP-1 cells, using YKL-40/CHI3L1 Rabbit mAb (A20792) at1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - YKL-40/CHI3L1 Rabbit mAb (A20792)}

    Western blot - YKL-40/CHI3L1 Rabbit mAb (A20792)

    Western blot analysis of various lysates using YKL-40/CHI3L1 Rabbit mAb (A20792) at1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - YKL-40/CHI3L1 Rabbit mAb (A20792)}

    Immunofluorescence - YKL-40/CHI3L1 Rabbit mAb (A20792)

    Confocal imaging of THP-1 cells using YKL-40/CHI3L1 Rabbit mAb (A20792, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - YKL-40/CHI3L1 Rabbit mAb (A20792)}

    Immunofluorescence - YKL-40/CHI3L1 Rabbit mAb (A20792)

    Confocal imaging of paraffin-embedded Rat brain tissue using YKL-40/CHI3L1 Rabbit mAb (A20792, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20792? Please let us know so that we can cite the reference in this datasheet.

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    Secondary Antibodies (26)