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YKT6 Rabbit pAb (A17083)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - YKT6 Rabbit pAb (A17083)

Western blot analysis of lysates from rat brain, using YKT6 Rabbit pAb (A17083) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameYKT6 Rabbit pAb
Catalog No.A17083
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene product is one of the SNARE recognition molecules implicated in vesicular transport between secretory compartments. It is a membrane associated, isoprenylated protein that functions at the endoplasmic reticulum-Golgi transport step. This protein is highly conserved from yeast to human and can functionally complement the loss of the yeast homolog in the yeast secretory pathway.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-198 of human YKT6 (NP_006546.1).
SequenceEFSKQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDETKIILHNTMESLLERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM
Gene ID
Swiss Prot
SynonymsYKT6
Calculated MW22kDa
Observed MW22kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat brain
Cellular locationapical dendrite, basal dendrite, cytoplasm, cytosol, endoplasmic reticulum, endosome, Golgi apparatus, mitochondrion, plasma membrane, SNARE complex
    ABclonal:Western blot - YKT6 Rabbit pAb (A17083)}

    Western blot - YKT6 Rabbit pAb (A17083)

    Western blot analysis of lysates from rat brain, using YKT6 Rabbit pAb (A17083) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (1)

    Secondary Antibodies (26)