제품 > 항체 > 다중클론항체(pAb)

YPEL5 Rabbit pAb (A14331)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - YPEL5 Rabbit pAb (A14331)

Western blot analysis of various lysates using YPEL5 Rabbit pAb (A14331) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameYPEL5 Rabbit pAb
Catalog No.A14331
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable metal ion binding activity. Predicted to be involved in cell population proliferation. Part of ubiquitin ligase complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-121 of human YPEL5 (NP_057145.1).
SequenceMGRIFLDHIGGTRLFSCANCDTILTNRSELISTRFTGATGRAFLFNKVVNLQYSEVQDRVMLTGRHMVRDVSCKNCNSKLGWIYEFATEDSQRYKEGRVILERALVRESEGFEEHVPSDNS
Gene ID
Swiss Prot
SynonymsCGI-127; YPEL5
Calculated MW14kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, HT-29, U-87MG, Mouse brain, Mouse lung, Mouse testis, Rat brain, Rat lung, Rat testis
Cellular locationextracellular region, microtubule organizing center, mitotic spindle pole, nucleus

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    ABclonal:Western blot - YPEL5 Rabbit pAb (A14331)}

    Western blot - YPEL5 Rabbit pAb (A14331)

    Western blot analysis of various lysates using YPEL5 Rabbit pAb (A14331) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)