제품 > 항체 > 다중클론항체(pAb)

YWHAH Rabbit pAb (A9079)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - YWHAH Rabbit pAb (A9079)

Western blot analysis of various lysates using YWHAH Rabbit pAb (A9079) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - YWHAH Rabbit pAb (A9079)

Immunofluorescence analysis of NIH-3T3 cells using YWHAH Rabbit pAb (A9079) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - YWHAH Rabbit pAb (A9079)

Immunofluorescence analysis of NIH-3T3 cells using YWHAH Rabbit pAb (A9079) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameYWHAH Rabbit pAb
Catalog No.A9079
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human YWHAH (NP_003396.1).
SequenceMGDREQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN
Gene ID
Swiss Prot
SynonymsYWHA1; YWHAH
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, LO2, Raji, Jurkat, Mouse heart, Mouse kidney
Cellular locationcerebellar granule cell to Purkinje cell synapse, cytoplasm, cytosol, extracellular exosome, plasma membrane, presynapse

Documents

Certificate of Compliance

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ABclonal:Western blot - YWHAH Rabbit pAb (A9079)}

Western blot - YWHAH Rabbit pAb (A9079)

Western blot analysis of various lysates using YWHAH Rabbit pAb (A9079) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - YWHAH Rabbit pAb (A9079)}

Immunofluorescence - YWHAH Rabbit pAb (A9079)

Immunofluorescence analysis of NIH-3T3 cells using YWHAH Rabbit pAb (A9079) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - YWHAH Rabbit pAb (A9079)}

Immunofluorescence - YWHAH Rabbit pAb (A9079)

Immunofluorescence analysis of NIH-3T3 cells using YWHAH Rabbit pAb (A9079) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)