제품 > 항체 > 다중클론항체(pAb)

YY2 Rabbit pAb (A16621)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - YY2 Rabbit pAb (A16621)

Western blot analysis of lysates from rat brain, using YY2 Rabbit pAb (A16621) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - YY2 Rabbit pAb (A16621)

Immunofluorescence analysis of NIH/3T3 cells using YY2 Rabbit pAb (A16621) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - YY2 Rabbit pAb (A16621)

Immunofluorescence analysis of HeLa cells using YY2 Rabbit pAb (A16621) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - YY2 Rabbit pAb (A16621)

Immunofluorescence analysis of C6 cells using YY2 Rabbit pAb (A16621) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameYY2 Rabbit pAb
Catalog No.A16621
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transcription factor that includes several Kruppel-like zinc fingers in its C-terminal region. It possesses both activation and repression domains, and it can therefore have both positive and negative effects on the transcription of target genes. This gene has an intronless coding region, and it appears to have arisen by retrotransposition of the related YY1 transcription factor gene, which is located on chromosome 14.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human YY2 (NP_996806.2).
SequenceGEGQAENPPDYSEYLKGKKLPPGGLPGIDLSDPKQLAEFTKVKPKRSKGEPPKTVPCSYSGCEKMFRDYAAMRKHLHIHGPRVHVCAECGKAFLESSKLRR
Gene ID
Swiss Prot
SynonymsZNF631; YY2
Calculated MW41kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRat brain
Cellular locationNucleus
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - YY2 Rabbit pAb (A16621)}

Western blot - YY2 Rabbit pAb (A16621)

Western blot analysis of lysates from rat brain, using YY2 Rabbit pAb (A16621) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - YY2 Rabbit pAb (A16621)}

Immunofluorescence - YY2 Rabbit pAb (A16621)

Immunofluorescence analysis of NIH/3T3 cells using YY2 Rabbit pAb (A16621) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - YY2 Rabbit pAb (A16621)}

Immunofluorescence - YY2 Rabbit pAb (A16621)

Immunofluorescence analysis of HeLa cells using YY2 Rabbit pAb (A16621) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - YY2 Rabbit pAb (A16621)}

Immunofluorescence - YY2 Rabbit pAb (A16621)

Immunofluorescence analysis of C6 cells using YY2 Rabbit pAb (A16621) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)