제품 > 항체 > 다중클론항체(pAb)

ZBED1 Rabbit pAb (A6792)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ZBED1 Rabbit pAb (A6792)

Western blot analysis of bewo, using ZBED1 Rabbit pAb (A6792) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - ZBED1 Rabbit pAb (A6792)

Immunofluorescence analysis of U-2 OS cells using ZBED1 antibody (A6792) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameZBED1 Rabbit pAb
Catalog No.A6792
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZBED1 (NP_001164607.1).
SequenceMENKSLESSQTDLKLVAHPRAKSKVWKYFGFDTNAEGCILQWKKIYCRICMAQIAYSGNTSNLSYHLEKNHPEEFCEFVKSNTEQMREAFATAFSKLKPESSQQPGQDALAVKAGHGYDSKKQQELTAAVLGLICEGLYPASIVDEPTFKVLLKTADPRYELPSRKYISTKAIPEKYGAVREVILKELAEATWCGISTDMWRSENQNRAYVTLAAHFLGLGAPNCLSMGSRCLKTFEVPEENTAETITRVLYEVFIEWGISAKVFGATTNYGKDIVKACS
Gene ID
Swiss Prot
SynonymsALTE; DREF; TRAMP; hDREF
Calculated MW78kDa
Observed MW 78kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesbewo
Cellular locationNucleus

Documents

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ABclonal:Western blot - ZBED1 Rabbit pAb (A6792)}

Western blot - ZBED1 Rabbit pAb (A6792)

Western blot analysis of bewo, using ZBED1 Rabbit pAb (A6792) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - ZBED1 Rabbit pAb (A6792)}

Immunofluorescence - ZBED1 Rabbit pAb (A6792)

Immunofluorescence analysis of U-2 OS cells using ZBED1 antibody (A6792) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)