제품 > 항체 > 다중클론항체(pAb)

ZC3H7A Rabbit pAb (A13190)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ZC3H7A Rabbit pAb (A13190)

Western blot analysis of various lysates using ZC3H7A Rabbit pAb (A13190) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - ZC3H7A Rabbit pAb (A13190)

Immunofluorescence analysis of C6 cells using ZC3H7A Rabbit pAb (A13190) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ZC3H7A Rabbit pAb (A13190)

Immunofluorescence analysis of HeLa cells using ZC3H7A Rabbit pAb (A13190) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ZC3H7A Rabbit pAb (A13190)

Immunofluorescence analysis of L929 cells using ZC3H7A Rabbit pAb (A13190) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameZC3H7A Rabbit pAb
Catalog No.A13190
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables miRNA binding activity. Involved in production of miRNAs involved in gene silencing by miRNA. Predicted to be located in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ZC3H7A (NP_054872.2).
SequenceMSNVSEERRKRQQNIKEGLQFIQSPLSYPGTQEQYAVYLRALVRNLFNEGNDVYREHDWNNSISQYTEALNIADYAKSEEILIPKEIIEKLYINRIACYSNMGFHDKVLEDCNIVLSLNASNCKALYRKSKALSDLGRYKKAYDAVAKCSLAVPQDEHVIKLTQELAQKLGFKIRKAYVRAELSLKSVPGDGATKALNHSVEDIEPDLLTPRQEAVPVVS
Gene ID
Swiss Prot
SynonymsZC3H7; HSPC055; ZC3HDC7; ZC3H7A
Calculated MW111kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesLO2, HeLa, A-375, K-562
Cellular locationNucleus

Documents

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ABclonal:Western blot - ZC3H7A Rabbit pAb (A13190)}

Western blot - ZC3H7A Rabbit pAb (A13190)

Western blot analysis of various lysates using ZC3H7A Rabbit pAb (A13190) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - ZC3H7A Rabbit pAb (A13190)}

Immunofluorescence - ZC3H7A Rabbit pAb (A13190)

Immunofluorescence analysis of C6 cells using ZC3H7A Rabbit pAb (A13190) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ZC3H7A Rabbit pAb (A13190)}

Immunofluorescence - ZC3H7A Rabbit pAb (A13190)

Immunofluorescence analysis of HeLa cells using ZC3H7A Rabbit pAb (A13190) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ZC3H7A Rabbit pAb (A13190)}

Immunofluorescence - ZC3H7A Rabbit pAb (A13190)

Immunofluorescence analysis of L929 cells using ZC3H7A Rabbit pAb (A13190) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)