제품 > 항체 > 다중클론항체(pAb)

ZCWPW1 Rabbit pAb (A7596)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ZCWPW1 Rabbit pAb (A7596)

Western blot analysis of extracts of various cell lines, using ZCWPW1 antibody (A7596) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameZCWPW1 Rabbit pAb
Catalog No.A7596
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables methyl-CpG binding activity and methylated histone binding activity. Predicted to be involved in meiosis I; positive regulation of DNA metabolic process; and spermatogenesis. Predicted to act upstream of or within homologous chromosome pairing at meiosis. Predicted to be located in XY body. Predicted to be active in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 389-648 of human ZCWPW1 (NP_060454.3).
SequenceVMKKRRNDCSQKLGVALMMAQEAEQISIQERVNLFGFWSRFNGSNSNGERKDLQLSGLNSPGSCLEKKEKEEELEKEEGEKTDPILPIRKRVKIQTQKTKPRGLGGDAGTADGRGRTLQRKIMKRSLGRKSTAPPAPRMGRKEGQGNSDSDQPGPKKKFKAPQSKALAASFSEGKEVRTVPKNLGLSACKGACPSSAKEEPRHREPLTQEAGSVPLEDEASSDLDLEQLMEDVGRELGQSGELQHSNSDGEDFPVALFGK
Gene ID
Swiss Prot
SynonymsZCW1
Calculated MW72kDa
Observed MW72kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesOVCAR3, SKOV3, mouse testis
Cellular locationnucleus
Customer validation

WB(Mus musculus)

Documents

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    ABclonal:Western blot - ZCWPW1 Rabbit pAb (A7596)}

    Western blot - ZCWPW1 Rabbit pAb (A7596)

    Western blot analysis of extracts of various cell lines, using ZCWPW1 antibody (A7596) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A7596? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)