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ZDHHC18 Rabbit pAb (A15199)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ZDHHC18 Rabbit pAb (A15199)

Western blot analysis of various lysates using ZDHHC18 Rabbit pAb (A15199) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - ZDHHC18 Rabbit pAb (A15199)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using ZDHHC18 Rabbit pAb (A15199) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameZDHHC18 Rabbit pAb
Catalog No.A15199
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables palmitoyltransferase activity. Involved in peptidyl-L-cysteine S-palmitoylation. Located in Golgi apparatus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 301-388 of human ZDHHC18 (NP_115659.1).
SequenceYLVASNLTTNEDIKGSWSSKRGGEASVNPYSHKSIITNCCAVLCGPLPPSLIDRRGFVQSDTVLPSPIRSDEPACRAKPDASMVGGHP
Gene ID
Swiss Prot
SynonymsDHHC18; DHHC-18; ZDHHC18
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRaji, HeLa, Mouse brain, Rat brain
Cellular locationMembrane, Multi-pass membrane protein.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - ZDHHC18 Rabbit pAb (A15199)}

    Western blot - ZDHHC18 Rabbit pAb (A15199)

    Western blot analysis of various lysates using ZDHHC18 Rabbit pAb (A15199) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - ZDHHC18 Rabbit pAb (A15199)}

    Immunohistochemistry - ZDHHC18 Rabbit pAb (A15199)

    Immunohistochemistry analysis of paraffin-embedded Human lung cancer using ZDHHC18 Rabbit pAb (A15199) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A15199? Please let us know so that we can cite the reference in this datasheet.