제품 > 항체 > 다중클론항체(pAb)

ZDHHC4 Rabbit pAb (A17980)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ZDHHC4 Rabbit pAb (A17980)

Western blot analysis of lysates from HeLa cells, using ZDHHC4 Rabbit pAb (A17980) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - ZDHHC4 Rabbit pAb (A17980)

Western blot analysis of lysates from Rat lung, using ZDHHC4 Rabbit pAb (A17980) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameZDHHC4 Rabbit pAb
Catalog No.A17980
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable protein-cysteine S-palmitoyltransferase activity. Predicted to be involved in peptidyl-L-cysteine S-palmitoylation and protein targeting to membrane. Located in Golgi apparatus and endoplasmic reticulum.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 110-210 of human ZDHHC4 (NP_060576.1).
SequenceVNLFFFTLTCGTNPGIITKANELLFLHVYEFDEVMFPKNVRCSTCDLRKPARSKHCSVCNWCVHRFDHHCVWVNNCIGAWNIRYFLIYVLTLTASAATVAI
Gene ID
Swiss Prot
SynonymsZNF374; ZDHHC4
Calculated MW40kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Rat lung
Cellular locationendoplasmic reticulum, Golgi apparatus, plasma membrane

Documents

Certificate of Compliance

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    ABclonal:Western blot - ZDHHC4 Rabbit pAb (A17980)}

    Western blot - ZDHHC4 Rabbit pAb (A17980)

    Western blot analysis of lysates from HeLa cells, using ZDHHC4 Rabbit pAb (A17980) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - ZDHHC4 Rabbit pAb (A17980)}

    Western blot - ZDHHC4 Rabbit pAb (A17980)

    Western blot analysis of lysates from Rat lung, using ZDHHC4 Rabbit pAb (A17980) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)