제품 > 항체 > 다중클론항체(pAb)

ZFPM2 Rabbit pAb (A9868)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ZFPM2 Rabbit pAb (A9868)

Western blot analysis of extracts of various cell lines, using ZFPM2 antibody (A9868) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.

Overview

Product nameZFPM2 Rabbit pAb
Catalog No.A9868
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The zinc finger protein encoded by this gene is a widely expressed member of the FOG family of transcription factors. The family members modulate the activity of GATA family proteins, which are important regulators of hematopoiesis and cardiogenesis in mammals. It has been demonstrated that the protein can both activate and down-regulate expression of GATA-target genes, suggesting different modulation in different promoter contexts. A related mRNA suggests an alternatively spliced product but this information is not yet fully supported by the sequence.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 982-1151 of human ZFPM2 (NP_036214.2).
SequenceSPYYGIKPSDYISGSLVIHNTDIEQSRNAENESPKGQASSNGCAALKKDSLPLLPKNRGMVIVNGGLKQDERPAANPQQENISQNPQHEDDHKSPSWISENPLAANENVSPGIPSAEEQLSSIAKGVNGSSQAPTSGKYCRLCDIQFNNLSNFITHKKFYCSSHAAEHVK
Gene ID
Swiss Prot
SynonymsDIH3; FOG2; SRXY9; ZNF89B; hFOG-2; ZC2HC11B
Calculated MW128kDa
Observed MW150kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-1080, K-562, Mouse liver
Cellular locationNucleus

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ABclonal:Western blot - ZFPM2 Rabbit pAb (A9868)}

Western blot - ZFPM2 Rabbit pAb (A9868)

Western blot analysis of extracts of various cell lines, using ZFPM2 antibody (A9868) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)