제품 > 항체 > 다중클론항체(pAb)

ZFYVE16 Rabbit pAb (A13154)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ZFYVE16 Rabbit pAb (A13154)

Western blot analysis of lysates from HeLa cells, using ZFYVE16 Rabbit pAb (A13154) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameZFYVE16 Rabbit pAb
Catalog No.A13154
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable 1-phosphatidylinositol binding activity and phosphatidylinositol-3, 4, 5-trisphosphate binding activity. Predicted to be involved in endosomal transport and protein targeting to lysosome. Predicted to be located in cytosol and early endosome. Predicted to be active in early endosome membrane. Is expressed in genitourinary system. Orthologous to human ZFYVE16 (zinc finger FYVE-type containing 16).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of mouse ZFYVE16 (NP_775568.1).
SequenceMDSYFKAAVSGLDKLLDDFEQNPDKQDYLPDAYAFNQCSVSSESASPQLALLSKDQRCISTCASSEACCEDANETFLEGKIHEGLTSRPNEKNVAGLDLLSSVDASTSDEIQPSCMRRCSKPVCDLISDMGNLVHATNSEEDIKQLLPDDPKSSADTLITLDSSSVSEALTVSSVDCGSNAVREEQNNINAGIKNRDISIKELGVKVDMALFDSCKYNRTENLKDKIISNELETVDFDMPSVLMEQSSEMSNTKDNPQYKRLPCELLKDDGCLAEEKVAVAVNNTECLEEGGGSNTIAMP
Gene ID
Swiss Prot
SynonymsmKIAA0305; B130031L15; B130024H06Rik; ZFYVE16
Calculated MW166kDa
Observed MW169kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular locationCytoplasm, Early endosome membrane, Peripheral membrane protein
    ABclonal:Western blot - ZFYVE16 Rabbit pAb (A13154)}

    Western blot - ZFYVE16 Rabbit pAb (A13154)

    Western blot analysis of lysates from HeLa cells, using ZFYVE16 Rabbit pAb (A13154) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.