제품 > 항체 > 다중클론항체(pAb)

ZFYVE1 Rabbit pAb (A7527)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ZFYVE1 Rabbit pAb (A7527)

Western blot analysis of lysates from 293T cells, using ZFYVE1 Rabbit pAb (A7527) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameZFYVE1 Rabbit pAb
Catalog No.A7527
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate-containing membranes. This protein contains two zinc-binding FYVE domains in tandem and is reported to localize to the Golgi apparatus. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 500-735 of human ZFYVE1 (NP_067083.1).
SequenceAKYAWSGYVIECPNCGVVYRSRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGMNFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKH
Gene ID
Swiss Prot
SynonymsSR3; DFCP1; TAFF1; ZNFN2A1; PPP1R172; ZFYVE1
Calculated MW87kDa
Observed MW87kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular locationEndoplasmic reticulum, Golgi apparatus, Golgi stack.
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Homo sapiens)

IHC(Homo sapiens)

Co-IP(Mus musculus)

IF(Homo sapiens)

    ABclonal:Western blot - ZFYVE1 Rabbit pAb (A7527)}

    Western blot - ZFYVE1 Rabbit pAb (A7527)

    Western blot analysis of lysates from 293T cells, using ZFYVE1 Rabbit pAb (A7527) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A7527? Please let us know so that we can cite the reference in this datasheet.