제품 > 항체 > 다중클론항체(pAb)

ZFYVE1 Rabbit pAb (A7527)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ZFYVE1 Rabbit pAb (A7527)

Western blot analysis of lysates from 293T cells, using ZFYVE1 Rabbit pAb (A7527) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameZFYVE1 Rabbit pAb
Catalog No.A7527
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate-containing membranes. This protein contains two zinc-binding FYVE domains in tandem and is reported to localize to the Golgi apparatus. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 500-735 of human ZFYVE1 (NP_067083.1).
SequenceAKYAWSGYVIECPNCGVVYRSRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGMNFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKH
Gene ID
Swiss Prot
SynonymsSR3; DFCP1; TAFF1; ZNFN2A1; PPP1R172; ZFYVE1
Calculated MW87kDa
Observed MW87kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular locationEndoplasmic reticulum, Golgi apparatus, Golgi stack.
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Homo sapiens)

IHC(Homo sapiens)

Co-IP(Mus musculus)

IF(Homo sapiens)

    ABclonal:Western blot - ZFYVE1 Rabbit pAb (A7527)}

    Western blot - ZFYVE1 Rabbit pAb (A7527)

    Western blot analysis of lysates from 293T cells, using ZFYVE1 Rabbit pAb (A7527) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A7527? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)