제품 > 항체 > 다중클론항체(pAb)

ZIP4 Rabbit pAb (A20588)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Oryza sativa

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using ZIP4 Rabbit pAb (A20588) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product name	ZIP4 Rabbit pAb
Catalog No.	A20588
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Required for crossover formation, complete synapsis of homologous chromosomes and bivalent formation during meiosis. Is specific to recombination events resulting in interference-sensitive crossovers (class I meiotic crossover) and works cooperatively with MER3 to promote crossovers.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of Oryza sativa ZIP4 (Q5N829).
Sequence	MKISELSPEYREPPSHAGLIADLSKAVSDVESFAASATAPEKLAADLRRILTSLASAASSSSFTESLSVQIWRLGTRLWNAVVDRANSAALAGGPAALAVEAEIRQAAPELLLLAGIPNGVPSAAAKVASFFHRSGLAWLDLGRVDLASACFEKATPLVSAAATEDRGVLLELNLARARAASDAGDQALAVALLSRSKPL
Gene ID
Swiss Prot
Synonyms	ZIP4; OsZIP4; OsJ_04366; B1078G07.1
Calculated MW	103kDa
Observed MW	103kDa

Applications

Reactivity	Oryza sativa
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Spikes
Cellular location	nucleus