제품 > 항체 > 다중클론항체(pAb)

ZMAT2 Rabbit pAb (A14354)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ZMAT2 Rabbit pAb (A14354)

Western blot analysis of various lysates using ZMAT2 Rabbit pAb (A14354) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameZMAT2 Rabbit pAb
Catalog No.A14354
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable DNA binding activity and zinc ion binding activity. Involved in mRNA splicing, via spliceosome. Located in nucleus. Part of U2-type precatalytic spliceosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human ZMAT2 (NP_653324.1).
SequenceMASGSGTKNLDFRRKWDKDEYEKLAEKRLTEEREKKDGKPVQPVKRELLRHRDYKVDLESKLGKTIVITKTTPQSEMGGYYCNVCDCVVKDSINFLDHINGKKHQRNLGMSMRVERSTLDQVKKRFEVNKKKMEEKQKDYDFEERMKELREEEEKAKAYKKEKQKEKKRRAEEDLTFEEDDEMAAVMGFSGFGSTKKSY
Gene ID
Swiss Prot
SynonymsSnu23; Ptg-12; ZMAT2
Calculated MW24kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesLO2, Jurkat, HeLa, SH-SY5Y, MCF7, Mouse brain, Rat brain
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - ZMAT2 Rabbit pAb (A14354)}

    Western blot - ZMAT2 Rabbit pAb (A14354)

    Western blot analysis of various lysates using ZMAT2 Rabbit pAb (A14354) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A14354? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (25)