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ZNF10 Rabbit pAb (A25234)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ZNF10 Rabbit pAb (A25234)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ZNF10 using ZNF10 Rabbit pAb(A25234) at 1:1000 dilution. 
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameZNF10 Rabbit pAb
Catalog No.A25234
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene contains a C2H2 zinc finger, and has been shown to function as a transcriptional repressor. The Kruppel-associated box (KRAB) domain of this protein is found to be responsible for its transcriptional repression activity. RING finger containing protein TIF1 was reported to interact with the KRAB domain, and may serve as a mediator for the repression activity of this protein.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 474-573 of human ZNF10 (NP_056209.2).
SequenceSALIVHQRIHTGEKPYECCQCGKAFIRKNDLIKHQRIHVGEETYKCNQCGIIFSQNSPFIVHQIAHTGEQFLTCNQCGTALVNTSNLIGYQTNHIRENAY
Gene ID
Swiss Prot
SynonymsKOX1; ZNF10
Calculated MW66kDa
Observed MW66kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T transfected with ZNF10
Cellular locationNucleus

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ABclonal:Western blot - ZNF10 Rabbit pAb (A25234)}

Western blot - ZNF10 Rabbit pAb (A25234)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ZNF10 using ZNF10 Rabbit pAb(A25234) at 1:1000 dilution. 
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)