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ZNF177 Rabbit pAb (A14802)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - ZNF177 Rabbit pAb (A14802)

Immunohistochemistry analysis of paraffin-embedded rat pancreas using ZNF177 Rabbit pAb (A14802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ZNF177 Rabbit pAb (A14802)

Immunohistochemistry analysis of paraffin-embedded mouse brain using ZNF177 Rabbit pAb (A14802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ZNF177 Rabbit pAb (A14802)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using ZNF177 Rabbit pAb (A14802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ZNF177 Rabbit pAb (A14802)

Immunohistochemistry analysis of paraffin-embedded mouse pancreas using ZNF177 Rabbit pAb (A14802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - ZNF177 Rabbit pAb (A14802)

Immunofluorescence analysis of C6 cells using ZNF177 Rabbit pAb (A14802) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ZNF177 Rabbit pAb (A14802)

Immunofluorescence analysis of NIH-3T3 cells using ZNF177 Rabbit pAb (A14802) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ZNF177 Rabbit pAb (A14802)

Immunofluorescence analysis of U-2 OS cells using ZNF177 Rabbit pAb (A14802) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameZNF177 Rabbit pAb
Catalog No.A14802
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II. Located in blood microparticle.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-110 of human ZNF177 (NP_003442.2).
SequenceLASVGYQLCRHSLISKVDQEQLKTDERGILQGDCADWETQLKPKDTIAMQNIPGGKTSNGI
Gene ID
Swiss Prot
SynonymsPIGX; ZNF177
Calculated MW55kDa
Observed MW
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationNucleus

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ABclonal:Immunohistochemistry - ZNF177 Rabbit pAb (A14802)}

Immunohistochemistry - ZNF177 Rabbit pAb (A14802)

Immunohistochemistry analysis of paraffin-embedded rat pancreas using ZNF177 Rabbit pAb (A14802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ZNF177 Rabbit pAb (A14802)}

Immunohistochemistry - ZNF177 Rabbit pAb (A14802)

Immunohistochemistry analysis of paraffin-embedded mouse brain using ZNF177 Rabbit pAb (A14802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ZNF177 Rabbit pAb (A14802)}

Immunohistochemistry - ZNF177 Rabbit pAb (A14802)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using ZNF177 Rabbit pAb (A14802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ZNF177 Rabbit pAb (A14802)}

Immunohistochemistry - ZNF177 Rabbit pAb (A14802)

Immunohistochemistry analysis of paraffin-embedded mouse pancreas using ZNF177 Rabbit pAb (A14802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - ZNF177 Rabbit pAb (A14802)}

Immunofluorescence - ZNF177 Rabbit pAb (A14802)

Immunofluorescence analysis of C6 cells using ZNF177 Rabbit pAb (A14802) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ZNF177 Rabbit pAb (A14802)}

Immunofluorescence - ZNF177 Rabbit pAb (A14802)

Immunofluorescence analysis of NIH-3T3 cells using ZNF177 Rabbit pAb (A14802) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ZNF177 Rabbit pAb (A14802)}

Immunofluorescence - ZNF177 Rabbit pAb (A14802)

Immunofluorescence analysis of U-2 OS cells using ZNF177 Rabbit pAb (A14802) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)