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ZNF259 Rabbit mAb (A1487)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ZNF259 Rabbit mAb (A1487)

Western blot analysis of lysates from Mouse brain, using ZNF259 Rabbit mAb (A1487) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ZNF259 Rabbit mAb (A1487)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using ZNF259 Rabbit mAb (A1487) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameZNF259 Rabbit mAb
Catalog No.A1487
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2563
The protein encoded by this gene is found in the cytoplasm of quiescent cells but translocates to the nucleolus in proliferating cells. The encoded protein interacts with survival motor neuron protein (SMN1) to enhance pre-mRNA splicing and to induce neuronal differentiation and axonal growth. Defects in this gene or the SMN1 gene can cause spinal muscular atrophy. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 360-459 of human ZNF259 (O75312).
SequenceDIRELVTKNPFTLGDSSNPGQTERLQEFSQKMDQIIEGNMKAHFIMDDPAGNSYLQNVYAPEDDPEMKVERYKRTFDQNEELGLNDMKTEGYEAGLAPQR
Gene ID
Swiss Prot
SynonymsGKAF; ZNF259
Calculated MW51kDa
Observed MW51kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain
Cellular locationCajal body, Cell projection, Cytoplasm, Nucleus, axon, gem, growth cone, nucleolus, perinuclear region

Documents

Certificate of Compliance

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    ABclonal:Western blot - ZNF259 Rabbit mAb (A1487)}

    Western blot - ZNF259 Rabbit mAb (A1487)

    Western blot analysis of lysates from Mouse brain, using ZNF259 Rabbit mAb (A1487) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - ZNF259 Rabbit mAb (A1487)}

    Immunohistochemistry - ZNF259 Rabbit mAb (A1487)

    Immunohistochemistry analysis of paraffin-embedded human liver cancer using ZNF259 Rabbit mAb (A1487) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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