제품 > 항체 > 다중클론항체(pAb)

ZNF350 Rabbit pAb (A7829)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ZNF350 Rabbit pAb (A7829)

Western blot analysis of Jurkat, using ZNF350 Rabbit pAb (A7829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameZNF350 Rabbit pAb
Catalog No.A7829
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding activity. Involved in negative regulation of transcription by RNA polymerase II. Located in nuclear body. Part of transcription repressor complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 285-532 of human ZNF350 (NP_067645.3).
SequenceTGEKPYICSECGKGFIQKGNLIVHQRIHTGEKPYICNECGKGFIQKTCLIAHQRFHTGKTPFVCSECGKSCSQKSGLIKHQRIHTGEKPFECSECGKAFSTKQKLIVHQRTHTGERPYGCNECGKAFAYMSCLVKHKRIHTREKQEAAKVENPPAERHSSLHTSDVMQEKNSANGATTQVPSVAPQTSLNISGLLANRNVVLVGQPVVRCAASGDNRGFAQDRNLVNAVNVVVPSVINYVLFYVTENP
Gene ID
Swiss Prot
SynonymsZFQR; ZBRK1
Calculated MW60kDa
Observed MW60kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat
Cellular locationNucleus, Nucleus matrix

Documents

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ABclonal:Western blot - ZNF350 Rabbit pAb (A7829)}

Western blot - ZNF350 Rabbit pAb (A7829)

Western blot analysis of Jurkat, using ZNF350 Rabbit pAb (A7829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)