제품 > 항체 > 다중클론항체(pAb)

ZNF521 Rabbit pAb (A20646)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - ZNF521 Rabbit pAb (A20646)

Western blot analysis of lysates from Rat brain, using ZNF521 Rabbit pAb (A20646) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameZNF521 Rabbit pAb
Catalog No.A20646
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables protein domain specific binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within neuron fate commitment. Located in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 210-420 of human ZNF521 (NP_056276.1).
SequenceGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCVYCHELFVEETSLMNHMEQVHSGEKKNSCSICSESFHTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTGPSSKQAKVTYSCIYCNKQLFSSLAV
Gene ID
Swiss Prot
SynonymsEHZF; Evi3; ZNF521
Calculated MW148kDa
Observed MW148kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat brain
Cellular locationnucleoplasm, nucleus
ABclonal:Western blot - ZNF521 Rabbit pAb (A20646)}

Western blot - ZNF521 Rabbit pAb (A20646)

Western blot analysis of lysates from Rat brain, using ZNF521 Rabbit pAb (A20646) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)