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ZNF574 Rabbit pAb (A14923)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - ZNF574 Rabbit pAb (A14923)

Immunohistochemistry analysis of paraffin-embedded human stomach using ZNF574 Rabbit pAb (A14923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ZNF574 Rabbit pAb (A14923)

Immunohistochemistry analysis of paraffin-embedded mouse pancreas using ZNF574 Rabbit pAb (A14923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - ZNF574 Rabbit pAb (A14923)

Immunofluorescence analysis of NIH-3T3 cells using ZNF574 Rabbit pAb (A14923) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ZNF574 Rabbit pAb (A14923)

Immunofluorescence analysis of U-2 OS cells using ZNF574 Rabbit pAb (A14923) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameZNF574 Rabbit pAb
Catalog No.A14923
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 170-310 of human ZNF574 (NP_073589.4).
SequencePPVGQARVAVEHSYRKAEEGGEGATVPSAAATTTEVVTEVELLLYKCSECSQLFQLPADFLEHQATHFPAPVPESQEPALQQEVQASSPAEVPVSQPDPLPASDHSYELRNGEAIGRDRRGRRARRNNSGEAGGAATQELF
Gene ID
Swiss Prot
SynonymsFP972; ZNF574
Calculated MW99kDa
Observed MW
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationNucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunohistochemistry - ZNF574 Rabbit pAb (A14923)}

    Immunohistochemistry - ZNF574 Rabbit pAb (A14923)

    Immunohistochemistry analysis of paraffin-embedded human stomach using ZNF574 Rabbit pAb (A14923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - ZNF574 Rabbit pAb (A14923)}

    Immunohistochemistry - ZNF574 Rabbit pAb (A14923)

    Immunohistochemistry analysis of paraffin-embedded mouse pancreas using ZNF574 Rabbit pAb (A14923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - ZNF574 Rabbit pAb (A14923)}

    Immunofluorescence - ZNF574 Rabbit pAb (A14923)

    Immunofluorescence analysis of NIH-3T3 cells using ZNF574 Rabbit pAb (A14923) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ZNF574 Rabbit pAb (A14923)}

    Immunofluorescence - ZNF574 Rabbit pAb (A14923)

    Immunofluorescence analysis of U-2 OS cells using ZNF574 Rabbit pAb (A14923) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)