제품 > 항체 > 다중클론항체(pAb)

ZNF597 Rabbit pAb (A14452)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ZNF597 Rabbit pAb (A14452)

Western blot analysis of various lysates using ZNF597 Rabbit pAb (A14452) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ZNF597 Rabbit pAb (A14452)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using ZNF597 Rabbit pAb (A14452) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameZNF597 Rabbit pAb
Catalog No.A14452
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein with multiple zinc finger domains. Loss of the related gene in rodents results in defects in neural development and embryonic lethality in mutant homozygotes. This gene is adjacent to a differentially methylated region (DMR) and is imprinted and maternally expressed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ZNF597 (NP_689670.1).
SequenceMASMPPTPEAQGPILFEDLAVYFSQEECVTLHPAQRSLSKDGTKESLEDAALMGEEGKPEINQQLSLESMELDELALEKYPIAAPLVPYPEKSSEDGVGNPEAKILSGTPTYKRRVISLLVTIENHTPLVELSEYLGTNT
Gene ID
Swiss Prot
SynonymsHIT-4; ZNF597
Calculated MW48kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, Jurkat, HeLa, U-87MG
Cellular locationNucleus

Documents

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ABclonal:Western blot - ZNF597 Rabbit pAb (A14452)}

Western blot - ZNF597 Rabbit pAb (A14452)

Western blot analysis of various lysates using ZNF597 Rabbit pAb (A14452) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ZNF597 Rabbit pAb (A14452)}

Immunohistochemistry - ZNF597 Rabbit pAb (A14452)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using ZNF597 Rabbit pAb (A14452) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.